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KMID : 0903619830240020135
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Journal of the Korean Society for Horticultural Science
1983 Volume.24 No. 2 p.135 ~ p.143
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Studies on the Shoot Tip culture of M. 7, M. 26, MM. 106 Apple Rootstock
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Abstract
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This study was carried cut to establish the methods cf rapid propagation of M.7, M.26, MM.106 apple rootstocks by shoot tip cultures. results were summarized as follows.
1. Apple shoots were readily damaged by common surface sterilants. In this study, the following procedure provided undamaged sterile shoot tips : the terminal 2-4cm portions of the shoots were collected into distilled water and then immersed momenterily in 0.01% Tween 20 and immediately afterwards in sodium hypocholorite solution (0.4% w/v available Cl) for 10 minutes, then followed by a rinse of 70% ethanol and 3 washes of sterile distilled water. It was possible to acquire undamaged sterile shoot tips of 80% with this sterilization procedure.
2. The optimum time for shoot collection was mid-May.
3. Two media were selected for shooting: The first was AIS(Revised) formulation supplemented with 1 ㎎/ℓ BA, 1 ㎎/ℓ IBA and 0.1 ㎎/ℓ GA₃. The second was MS (Revised) formulation sioplemented with 1 ㎎/ℓ Ba. and 1 ㎎/ℓ IBA.
4. A 6 fold shoot multiplication rate per 6 weeks was achieved with secondary subculture and there were no differences in shoot multiplication rate among 3 apple rootstocks.
5. The best medium far rooting was 1/3 strength of MS(Revised) formulation supplemented with 1 ㎎/ℓ IBA and 0.1 ㎎/ℓ GA₃. In all 3 apple rootstocks, about 60∼80% of shoots were rooted well on this medium within 6 weeks.
6. In this study, it seemed that phloroglucinol was ineffective in both shooting and rooting.
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